Guide to cytology

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Gelatinous cube living 64px.gif
 
Gelatinous Cube says:
"Why are they welding the vents, father? I didn't even eat that many of them."

This is the guide to cytology, science of living cells. Understanding cytology will help you grow a whole ass animal from as little as a single cell.

Getting started

In order to grow a creature we need a sample of cells, a reagent mixture to grow the creature in and a special growing vat.

Biopsy Tool Hunting for cells

The first and most straightforward way of doing this is using your biopsy tool on a creature, if it is able to be cultivated, a green light on the tool will indicate you have collected a viable cell sample. The drawback of this is of course you have to obtain a living or dead specimen of the creature you want to create.

The second and perhaps more exciting method is using your sterile swabs to swab objects for cell samples. A successful swab will often yield a mixture of cell lines from different organisms.

Thanks to the excellent janitorial staff aboard the station, most objects are quite clean and thus unfit for this purpose. Fortunately enough, the grimy, cold and dank maintenance shafts provide the perfect environment to hunt for biological samples.

Try swabbing anything that looks gross. There is a good chance such objects are covered in precious biofilm, a thin coat of microorganisms as well as cells from various earth and space creatures.

When you swab a piece of maintenance filth and a splotch of biofilm collects on the swab, you know you have done it right, because this means you have a mixed cell sample, ready for cultivation in the lab.

What follows is a list of the broader categories of sample-rich filth and garbage you can find. Most of these spawn at the start of the round, so collect your samples early, lest the janitor scrub them clean!

Sludge Decomposing Garbage, Old Vomit, Old Rotting Gibs, Trash Carts
Moisture Moisture Traps
Mold Dirty Matresses, Moldy Bread, Moldy Pizza, Moldy Messes
Algae Neglected fern (Rare chance to find in maintenance)

Microscope Devising a culture medium

Once you have collected a sample, deposit it into a petri-dish.

Now we need to figure out what kind of cells we have, and how to grow them. To do just this our lab is equipped with a microscope.

When a dish is inserted into the microscope, it tells us what kind of cells we have and three lists of reagents for each cell type.

  • Required reagents; we need all these reagents for the specimen to grow at all, it takes at least 10u of these to grow the specimen to completion, more if other cells compete for the same reagent.
  • Complementary reagents; each of these helps the cell line grow, but are not strictly necessary, but you might want to add some of these if you have mixture of cells to ensure other cell lines does not outgrow your target cell lines.
  • Suppressive reagents; these chemicals inhibit growth, or even kill off, this type of cells. You want to avoid contaminating your growing broth with reagents that suppress your target cell line, but they are very useful for suppressing undesired cell lines when growing a creature from a mixed sample.

Once you collect the reagents needed to grow your specimen we can move on to the next stage.

Research Plumbing Device Growing your specimen

Use your research plumbing constructor to create (and wrench) a growing vat. This is a plumbing connectable machine which you can fill with your cultivation broth. To fill a growing vat with reagents you must connect it to a wrenched down input gate, and then fill the input gate with your mix of reagents. See the Guide to plumbing for details about how to use a plumbing constructor. Once you have filled the vat with your medium, simply take your petri-dish and pour in some of your cell sample into the vat. If the nutritional requirements of any of the cell lines are met, the cultivation medium should start to bubble.

After some time, if you got your cultivation medium right, your target organism should emerge from the growing vat. If you screwed up, you might receive the wrong type of creature, a bloody mess or nothing at all!

Plunger.png To empty a growing vat use a plunger on it.

How do I get chemicals I need?

It is worth noting that many of the reagents you need have to be liberated from the item that contains them by grinding. Since our vat is a plumbing machine, we can easily use the grinder, filter and tank plumbing machines to process and store our reagents.

Protein

Protein, a class of biopolymers made up of amino acids, is a vital component of most known forms of life. This reagent is needed to grow most mobs, and having a way to obtain it is important.

The main source of this reagent is meat, and if you just want to grow a few specimens, picking the kitchen counter clean might be enough. However if you undertake a larger scale research project, you may need to obtain a more reliable source.

Butchering animals and grinding the resultant meat slabs will yield some protein: 6u per slab. Butchering animals also provides you with liquid gibs and occasionally other useful products. Some good ways of obtaining animals include:

  • Breeding mobs such as chickens, mice, cats or corgis.
  • Monkey cubes (inject grey slime extracts with blood to get a lot of these)

If you have access to a biogenerator you can mass-produce tofu, which contains 2u of protein apiece. Grinding tofu results in pure protein, meaning that you don't have to separate the protein out with a ChemMaster like you would have to with meat, and you don't have to go through the chore of butchering and sifting through piles of protein-free organs. It can be useful to request access to hydroponics for this very reason.

Other reagents

This is the tricky part, the other reagents are produced from a variety of sources around the station.

Many products of the hydroponics lab contain reagents you want, so for things to go smoothly you might want to some connections with the botanists or relocate a plant tray to your lab.

What is the point of all this?

Currently, this mechanic serves as a way to create powerful creatures to treat with a sentience potion as well as a source of exotic meats. The feature is still in development and in the future, this technology could be used to create feed animals for other creatures, chimaeras or useful items.

Cell Line Tables

Animals/Vertebrates

Cell Line Creates Growth Rate Viral Susceptibility Required Chemical Supplementary Reagents Suppressive Reagents Source
Murine Cells Mouse (x2) 4 2 Protein Growth Serum (+2)
Liquid Gibs (+2)
Corn Oil (+2)
Nutriment (+1)
Vitamin (+1)
Sugar (+1)
Cooking Oil (+1)
Rice (+1)
Egg Yolk (+1)
Heparin (-6)
Astrotame (-4)
Rubberneck (-3)
Grey Bull (-1)
Mice,
Dead Mice,
Sludge,
Mold
Galliform skin cells Chicken 4 1 Protein Rice (+4)
Growth Serum (+3)
Vitamin (+2)
Egg Yolk (+1)
Oil (-4)
Toxin (-2)
Chickens,
Chicken Meat,
Sludge
Bovine Stem Cells Cow 4 1 Protein,
Nutriment,
Cellulose
Growth Serum (+4)
Vitamin (+2)
Rice (+2)
Flour (+1)
Carpotoxin (-5)
Toxin (-2)
Cows,
Beef,
Sludge
Fairyland Bovine Stem Cells Moonicorn 4 1 Protein,
Nutriment,
Mushroom Hallucinogen
Growth Serum (+4)
Tinea Luxor (+2)
Vitfro (+2)
Astrotame (+1)
Toxin (-2)
Carpotoxin (-5)
Coffee (-3)
Triple Citrus (-5)
Moon Rock
Feliform Cells Cat 4 1.5 Protein,
Liquid Gibs
Growth Serum (+3)
Vitamin (+2)
Oculine (+2)
Milk (+1)
Coco Powder (-4)
Hot Coco (-2)
Chocolate Pudding (-2)
Chocolate Milk (-1)
Cats,
Mold
Canid Cells Corgi 4 1 Protein,

Liquid Gibs

Growth Serum (+3)
Barbers Aid (+3)
Vitamin (+2)
Tear Juice (-3)
Garlic (-2)
Coco Powder (-2)
Corgis,
Corgi Meat,
Mold
Squat Canid Cells Pug 4 3 Protein,
Liquid Gibs
Vitamin (+3)
Growth Serum (+2)
Tear Juice (-3)
Garlic (-2)
Coco Powder (-2)
Pugs,
Pug Meat
Ursine Cells Bear 4 2 Protein,
Liquid Gibs,
Synthflesh
Honey (+8)
Growth Serum (+5)
Morphine (+4)
Vitamin (+3)
Condensed Capsaicin (-4)
Carpotoxin (-2)
Insulin (-2)
Space Bears,
Bear Meat,
Mold
Cyprinid Cells Space Carp 4 2 Protein,
Nutriment
Corn Oil (+4)
Carpotoxin (+3)
Cooking Oil (+2)
Vitamin (+2)
Bungotoxin (-6)
Mercury (-4)
Oxygen (-3)
Space Carp,
Carp Fillets,
Carp Sashimi,
Moisture
Cartilaginous Cyprinid Cells Mega Space Carp
(Space Shark)
4 1 Protein,
Nutriment,
Synthflesh
Corn Oil (+4)
Growth Serum (+3)
Carpotoxin (+2)
Cooking Oil (+2)
Vitamin (+2)
Bungotoxin (-6)
Oxygen (-3)
Mega Space Carp,
Moisture
Ophidic Cells Snake 4 ? Protein,
Liquid Gibs
Growth Serum (+3)
Peptides (+3)
Egg Yolk (+2)
Vitamin (+2)
Corn Syrup (-6)
Sulfur (-3)
Snakes,
Moisture,
Algae
Blattodeoid Anthropod (sic) Cells Cockroach (x5) 4 0 Protein Organic Slurry (+4)
Growth Serum (+2)
Slime Jelly (+2)
Vitamin (+1)
Bug Spray (-4)
Poisonberry Juice (-4)
Pest Killer (-2)
Cockroaches,
Sludge,
Moisture,
Mold
Amphibian Cells Frog 4 0.5 Protein Ants (+3)
Egg White (+2)
Vitamin (+1)
Carpotoxin (-3)
Bungotoxin (-3)
Spore Toxin (-3)
Plant-B-Gone (-2)
Drying Agent (-2)
Mold (-2)
Toxin (-1)
Frog,
Algae
Atypical

Amphibian Cells

Leaper 4 ? Protein,
Ants,
Egg Yolk,
Synthflesh
Growth Serum (+4)
bLaSToFF (+3)
Space Drugs (+2)
Eggnog (+2)
Vanilla Powder (2)
Banana Juice (+1)
Vitamin (+1)
Cyanide (-5)
Mold (-2)
Spore Toxin (-1)
Leaper,
Leaper Sludge,
Leaper Bubble,
Algae
Aphid Cells Queen Bee 4 0 Protein,
Corn Syrup
Honey (+4)
Korta Nectar (+3)
Red Queen (+3)
Champagne (+2)
Sugar Rush (+2)
Sugar (+1)
Lemonade (+1)
Vitamin (+1)
Carpotoxin (-3)
Pest Killer (-2)
Potato Juice (-2)
Nicotine (-1)
Bee,
Queen Bee,
Algae
Pseudoarachnoid Cells Mega Arachnid 4 ? Protein,
Ants

Omnizine

Venom (+6)
Kronkaine (+4)
Peptides (+3)
Squirt Cider (+2)
Nutraslop (+2)
Vitamin (+1)
Plasma (+1)
Organ Tissue (+1)
Liquid Gibs (+1)
Universal Enzyme (+1)
Bug Spray (-3)
Nicotine (-1)
Pest Killer (-1)
Mega Arachnid,
Algae

Slimes, Oozes & Blobs

Cell Line Creates Growth Rate Viral Susceptibility Required Chemical Supplementary Reagents Suppressive Reagents Source
Slime Particles Slime 4 0 Protein Slime Jelly (+2)
Liquid Gibs (+2)
Universal Enzyme (+1)
Frost Oil (-4)
Cryostylane (-4)
Morphine (-2)
Ice (-2)
Slimes,
Sludge,
Mold,
Moisture
Immature Blob Spores Blob Spore (x2) 4 0 Protein Vitamin (+3)
Liquid Gibs (+2)
Sulfur (+2)
Tinea Luxor (-6)
Napalm (-4)
Blob Spores,
Former Blob-Infested Tiles,
Mold
Blobular Myocytes Blobbernaut 4 0 Protein,
Synthflesh,
Sulfur
Growth Serum (+3)
Vitamin (+2)
Liquid Gibs (+2)
Egg Yolk (+2)
Shambler's Juice (+1)
Tinea Luxor (-6) Blobbernauts,
Former Blob-Infested Tiles,
Sludge
Cubic Ooze Particles Gelatinous Cube 4 0 Protein,
Slime Jelly,
Organic Slurry,
Universal Enzyme
Hollow Water (+4)
Corn Syrup (+3)
Gold (+2)
Peptides (+2)
Potato Juice (+1)
Liquid Gibs (+1)
Vitamin (+1)
Mint Toxin (-3)
Frost Oil (-2)
Ice (-1)
Gelatinous Cubes,
Moisture
Globular Ooze Particles Sholean Grapes 4 0 Protein,
Slime Jelly,
Organic Slurry,
Vitrium Froth
Omnizine (+4)
Peptides (+3)
Corn Syrup (+2)
Squirt Cider (+2)
Doctor's Delight (+1)
Saline-Glucose Solution (+1)
Liquid Gibs (+1)
Vitamin (+1)
Carpotoxin (-3)
Coffee Grounds (-2)
Frost Oil (-2)
Ice (-1)
Sholean Grapes,
Sludge

Experimental Clowns

Cell Line Creates Growth Rate Viral Susceptibility Required Chemical Supplementary Reagents Suppressive Reagents Source
Clown Bits with Banana Chunks Clownana 4 0? Protein,
Banana Juice
Saltpetre (+4)
Ammonia (+3)
Carbon Dioxide (+3)
Pulped Banana Peel (+2)
Robust Harvest (+1)
Clown's Tears (-8)
Plant-B-Gone (-4)
Silencer (-3)
Nothing (-2)
Oil (-1)
Clown Masks,
Clown Shoes,
Clown Suits
Hyperadipogenic Clown Stem Cells Banana Glutton 4 0? Protein,
Banana Juice,
Synthflesh
Vanilla Pudding (+8)
Growth Serum (+6)
Peptides (+4)
Corn Oil (+3)
Cooking Oil (+1)
Space Cola (+1)
Clown's Tears(-8)
Mint Toxin (-6)
Silencer (-3)
Fernet (-3)
Lipolicide (-3)
Nothing (-2)
Bad Food (-1)
Clown Masks,
Clown Shoes,
Clown Suits,
Banana Glutton
Long Clown Bits Longface 4 0? Protein,
Banana Juice
Happiness (+5)
Mime's Bane (+4)
Laughter (+3)
Nitrous Oxide (+2)
Clown's Tears (-8)
Beepsky Smash (-3)
Silencer (-3)
Mute Toxin (-3)
Nothing (-2)
Sulfur (-1
Clown Masks,
Clown Shoes,
Clown Suits

Miscellaneous

Cell Line Creates Growth Rate Viral Susceptibility Required Chemical Supplementary Reagents Suppressive Reagents Source
Coniferous Plant Cells Pine Tree 4 1 Ammonia,
Ash,
Robust Harvest
Saltpetre (+5)
Carbon Dioxide (+2)
Nutriment (+2)
Space Cola (+2)
Holy Water (+2)
Water (+1)
Cellulose (+1)
Plant-B-Gone (-8) Living Pine Trees
Hypergenic Xenocytes Vat Beast 4 0.5 Protein,
Vitamin,
Peptides,
Liquid Electricity,
Growth Serum,
Organic Slurry
Rezadone (+3)
Entropic Polpnium (+3)
Red Queen (+2)
Peach Juice (+2)
Uranium (+1)
Liquid Gibs (+1)
Salt (-3)
Syriniver (-2)
Vat Beasts,
Mold
Aberrant Residue Random Netherworld Mob 4 0 Hollow Water,
Wizz Fizz,
Organic Slurry
Wittel (+10)
Protozine (+5)
Plasma Oxide (+3)
Chlorine Trifluoride (+1)
Peptides (-6)
Protein (-4)
Nutriment (-3)
Liquid Gibs (-2)
Netherworld Creatures,
Lavaland Geysers
Motile Fungal Hyphae Walking Mushroom 4 0 Protein Polonium (+6)
Corn Syrup (+3)
Mushroom Tea (+3)
Coffee Grounds (+2)
Nuka Cola (+2)
Mold (+2)
Sugar (+1)

Cellulose (+1)

Lead (-4)
Garlic (-3)
Plasma (-2)
Flash Powder (-2)
Pax (-2)
Copper (-1)
Huge Mushroom Slice,
Algae